Fig 2: Western blot validation and IHC analysis of synaptophysin and synaptotagmin-1.The representative western blots of ß-actin, albumin, calretinin, SYPH, SYT-1, Annexin A1, ApoA4, calnexin, GNAL, GFAP, H2B, HSP90AB1 and vimentin shown as the expression levels in the retinas (A) and as the densitometric quantitative results (B). Equal amounts of protein from the control retinas and the I/R-treated retinas were loaded. Each lane represents individual retina (n = 4 or 6 in each group; *p<0.05 compared with the non-injured retinas). The error bars represent the standard error of the mean. C, Retinal I/R-induced reductions of the synaptic proteins SYPH and SYT-1. The representative western blots of synaptophysin (SYPH) and synaptotagmin-1 (SYT-1) shown as the expression levels in the retinas are seen in (A). The stained retinal sections of SYPH (B) and SYT-1 (C) are shown at 2 days after the injury. Blue color: DAPI stained nuclei as control. Red color: SYPH or SYT-1 positively stained synapses. The scale bar represents 50 µm. C, non-injured eyes; I/R, I/R-injured eyes.